Phytochemical analysis

In order to understand what phytochemicals are doing within a system, robust and quantitative methods are required for their analysis. We focus on developing liquid chromatography and mass spectrometry based methods to allow analysis of phytochemicals and their metabolites, often present at picomolar concentrations. Some papers here are also included which use our methods.

Here is some work we’ve published on the topic:

• Sholola MJ, Goggans ML, Dzakovich MP, Francis DM, Jacobi SK, Cooperstone JL. Discovery of steroidal alkaloid metabolites and their accumulation in pigs after short-term tomato consumption. bioRxiv, February 8, 2024.
• Thomas M, Puglisi M, Malysheva O, Caudill M, Sholola MJ, Cooperstone JL, Fernandez ML. Eggs improve plasma biomarkers in patients with metabolic syndrome following a plant-based diet. Nutrients, 2022;5:2138.
• Thomas MS, DiBella M, Blesso CN, Malisheva O, Caudill M, Sholola MJ, Cooperstone JL, Fernandez ML. Comparison between egg intake vs choline supplementation on gut microbiota and plasma carotenoids in subjects with metabolic syndrome. Nutrients, 2022;14(6):1179.
• Fenstemaker S, Sim L, Cooperstone JL, Francis DM. Solanum galapagense-derived purple tomato fruit color is conferred by novel alleles of the Anthocyanin fruit and atroviolacium loci. Plant Direct, 2022;6(4):e394.
  – Also published as a pre-print on bioRxiv.
• Sholola MJ, Cooperstone JL. Carotenoid extraction and analysis of blood plasma/serum. Methods in Enzymology 670: Carotenoid and Apocarotenoid Analysis. Ed. Eleanore Wurtzel. 2022;670;425-457.
• Dzakovich MP, Hartman JL, Cooperstone JL. A high-throughput extraction and analysis method for steroidal glycoalkaloids in tomato. Front Plant Sci, 2020;11:767.
  – Also published as a pre-print on bioRxiv.
• Dzakovich MP, Gas-Pascual E, Orchard CJ, Sari EN, Riedl KM, Schwartz SJ, Francis DM, Cooperstone JL. Analysis of tomato carotenoids: comparison extraction and chromatographic methods. J AOAC Int. 2019;102(4):1069-1079.
• Cooperstone JL, Novotny JA, Riedl KM, Cichon MJ, Francis DM, Curley Jr. RW, Schwartz SJ, Harrison EH. Limited appearance of apocarotenoids is observed in plasma after consumption of tomato juices, a randomized human clinical trial. Am J Clin Nutr. 2018;108:1-8.
• Cooperstone JL, Tober KA, Riedl KM, Teegarden MDg, Cichon MJ, Francis DM, Schwartz SJ, Oberyszyn TA. Tomatoes protect against development of UV-induced keratinocyte carcinoma via metabolomic alterations. Sci Reports, 2017;5:5106.
• Teegarden MD, Harper AR, Cooperstone JL, Tober KA, Schwartz SJ, Oberyszyn TA. 25-Hydroxyvitamin D3 and it’s C-3 epimer are elevated in the skin and serum of Skh-1 mice supplemented with dietary vitamin D3. Mol Nutr Food Res, 2017;61:1700293.
• Goetz HJ, Kopec RE, Riedl KM, Cooperstone JL, Narayanasamy S, Curley Jr., RW, Schwartz SJ. An HPLC-MS/MS method for the separation of α-retinyl esters from retinyl esters. J Chrom B, 2016;1029:68-71.
• Cooperstone JL, Francis DM, Schwartz SJ. Thermal processing differentially affects lycopene and other carotenoids in cis-lycopene containing, tangerine tomatoes. Food Chem 2016;210:466-472.
• Kopec RE, Cooperstone JL, Cichon MJ, Schwartz SJ. Analysis Methods of Carotenoids.In: Analysis of Antioxidant Rich Phytochemicals; Xu, Z., Howard, L.R. eds.; John Wiley & Sons: Hoboken, NJ, 2012. P. 105-148.
• Pumilia G, Cichon MJ, Cooperstone JL, Giuffrida D, Dugo G, Schwartz SJ. Changes in chlorophylls, chlorophyll degradation products and lutein in pistachio kernels (Pistachia vera L.) during roasting. Food Res Int, 2014;65B:193-198.

If you have trouble accessing any work, check here or send Jess an email and she’d be happy to share with you.