Metabolomics short course at Universidade Federal do Rio Grande do Sul (UFRGS) 🇧🇷 Summer 2024
Instructor: Jessica Cooperstone, Ph.D.
Associate Professor, Horticulture and Crop Science, Food Science and Technology
The Ohio State University
Zoom link: https://go.osu.edu/ufrgs
The purpose of this short course is to give students a conceptual and hands-on introduction to the metabolomics workflow, experimental design process, collection of data via LC combined with high resolution MS, data analysis process, and compound identification.
Below is an overview of the material to be covered in this workshop. The top toolbar has lecture material under the Lectures tab and Hands on activities under the Activities tab.
Introduction to metabolomics
Study design and sample collection
LC-MS data acquisition and pre-processing
- Basics of LC and MS
- Sample preparation
- Data collection
- Raw data processing to create a feature table using MZmine
Data analysis using MetaboAnalyst
- How to use MetaboAnalyst
- Looking at your data and assessing quality
- Creating principal components analysis (PCA) plots
- Univariate group difference testing
- Multivariate class discovery and prediction methods